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Figure 4 Time-lapse analysis of intracellular reactive oxygen species production in neonatal rat cardiac myocytes. (A) Representative sequential images of chloromethyl-2,7-dichlorodihydrofluorescein diacetate (DCF) fluorescence in each group. H = cells exposed to 50 µmol/l H₂O₂ for 30 min; SFA = cells pretreated with 100 µmol/l serofendic acid for 30 min followed by 50 µmol/l H₂O₂ for 30 min. Scale bars at 0 min frames: 20 µm. (B) Time course of DCF fluorescence of 25 cells randomly selected in each group. Similar results were obtained in three independent experiments. (C) Mean fluorescence intensity from 25 cells randomly and prospectively selected in each group. *p < 0.05 versus H at the end of the experimental period. Other abbreviations as in Figure 1. Please see the for an accompanying video to this figure.