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Figure 6 Photomicrographs showing immunofluorescent staining of a small artery and infiltrating cell within the infarct myocardium. (A) Combination staining of the target vessel with anti-placental growth factor (red) and anti-von Willebrand factor (green) antibodies. Yellow coloration indicates placental growth factor expression within vascular endothelial cells. (B) Combination staining with anti-placental growth factor (red) and anti-alpha-smooth muscle actin (green) antibodies. Note that placental growth factor expression is restricted to the intima of the target vessel. (C) Combination staining with anti-placental growth factor (red) and anti-CD11b (green) antibodies. Placental-growth-factor-expressing cell in the interstitium did not cross-react with CD11b-positive cell (macrophage). Original magnification, 800x. DAPI was used for nuclear staining (blue).