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Figure 5 Photomicrographs showing immunohistochemical staining of placental growth factor in mouse heart on day 3 post-myocardial infarction. (A) Infarct myocardium (original magnification, 60x). (B and C) Higher magnification (240x) of the corresponding box (A). The staining shows strong expression of placental growth factor protein in the endothelium within the infarct region (B), as well as in interstitial cells (C). The staining shows an absence of placental growth factor protein in the non-infarct area (D). Arrows indicate vascular tissue, arrowheads interstitial cells.