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Figure 4 Expression of placental growth factor (PlGF) mRNA in the hearts of mice subjected to myocardial infarction induced by coronary artery ligation. Quantitative real-time polymerase chain reaction revealed that expression of PlGF mRNA was significantly up-regulated in infarct hearts, as compared to sham-operated hearts on days 1 and 3 post-myocardial infarction, but had returned to levels that did not differ from control by days 7 and 28. AMI = acute myocardial infarction.