Click on image to view larger version.

Figure 5 Akt1 activity and vascular endothelial growth factor (VEGF) expression in myocardium. Whole tissue lysates were extracted from myocardium of sham-operated swine and ischemic myocardium of operated swine, and subjected to Western blot analysis. (A) The membranes were incubated with antibodies against Akt1 and phospho-Akt1. Results are representative of five independent experiments. (B) Quantitative analysis of Akt1 activity. Results are given as mean ± SEM. (C) A representative photograph of VEGF expression. Results are representative of three independent experiments. (D) Quantitative analysis of VEGF expression. Results are given as mean ± SEM. *p < 0.05.