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Figure 4 Apoptosis of ischemic myocardium. Apoptotic cells of ischemic area at eight weeks after the operation were identified by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-digoxigenin nick end labeling (TUNEL) staining. (A) A representative photograph of TUNEL-positive cells. Bar indicates 30 µm. (B) The percentage of the number of apoptotic cells in total cells. (C) Representative photographs of von Willebrand factor (vWF)- and TUNEL-positive cells. Bar indicates 50 µm. (D) The percentage of vWF- and TUNEL-positive cells to total TUNEL-positive cells. Results are given as mean ± SEM. *p < 0.05.