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Figure 4 Oxidized nuclear and mitochondrial DNA levels. (A) mtDNA isolated and digested with BamH1 restriction enzyme, which results in two bands of approximately 8 and 9 kbp, indicating the purity of the isolated mtDNA. (B) Immuno-slot blot of mtDNA (upper blot) and the corresponding ethidium bromide stained blot (lower blot) in three mice in each of the experimental groups. (C) Immuno-slot blot (upper blot) of nuclear DNA and ethidium bromide stained blot (lower blot) in three mice per experimental group.