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Figure 8 Quantifying graft male mesenchymal stem cells (MSCs) in the female ischemic myocardium by real-time polymerase chain reaction. (A) Real-time amplification plot showing change in normalized reporter dye fluorescence (Rn) versus number of amplification cycles in sample containing serially diluted male MSC genomic DNA. (B) Standard curve generated from data in (a) showing relationship between threshold cycle (Ct) and number of male MSCs. (C) Time course of graft MSC survival (%). The survival for MSC_HO-1 was approximately 68% at 4 h and further decreased to 18% at seven days; however, the survival for MSC_LacZ decreased from 21% to 3.6% for this time period.