Click on image to view larger version.

Figure 6 Detection of inflammatory cytokine by Western blot and inflammatory cell infiltration by CD45 staining in ischemic myocardium. (A) and (B) Down-regulation of interleukin-1ß was detected in heme oxygenase-1 plasmid-treated ischemic myocardium one day after treatment, whereas an obvious elevation of interleukin-1b in LacZ plasmid-treated hearts was found. Data were shown as mean ± SE after normalization with glyseraldehyde-3-phosphate dehydrogenase and compared by Student t test, _*p = 0.045 compared with LacZ. (C) and (D) Immunohistochemical staining to detect the CD45 cells in ischemic myocardium. The number of CD45-positive cells was decreased in HO-1 plasmid-treated myocardium four days after treatment. Arrows indicate the groups of CD45-positive cells.