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Figure 1 Diagram of hypoxia-regulated plasmid system, which can amplify the power of a promoter based on the strong transcription activity of the GAL4/p65 fusion protein. The ischemic biosensor is composed of an oxygen-sensing toggle (OST), which is a GAL4DNA-binding domain, ODD of the hypoxia-inducible factor-1-alpha along with p65 activation domain. The effector plasmid contains GAL4 upstream activation sequence (UAS) in front of an adenovirus E1b TATA box and the hHO-1-6xHis or LacZ fusion gene. Hypoxia inducible LacZ system is replaced hHO-1 gene with LacZ.