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Figure 7 TaqMan polymerase chain reaction (PCR) showing messenger ribonucleic acid (mRNA) expression (left) of stromal cell-derived factor-1 (SDF-1) in the control (upper panel) and ischemia (lower panel) groups with the corresponding ribosomal ribonucleic acid (rRNA) (right) as internal control. The ischemia group demonstrates a shift of the mRNA curve to the left, indicating an earlier threshold of detection on the PCR thermal cycle and thus an increased mRNA expression of SDF-1. Rn = change in ribonucleic acid acitivity.