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Figure 3 (A) Representative Western blot showing phospho-flk-1 and Akt and endothelial nitric oxide synthase (eNOS) protein expression and their phosphorylation forms in the myocardial samples from the three groups included in the study. 1 to 3 = control specimens; 4 to 6 = non-diabetic patients with chronic CHD; 7 to 9 = diabetic patients with chronic CHD. (B) Optical density (O.D.) of phospho-flk-1, phospho-Akt, total and phospho-eNOS, and phospho-eNOS/total eNOS ratio. *Statistical difference from control specimens; **statistical difference from non-diabetic patients. Other abbreviations as in Figure 1.