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Figure 3 Western blots probed using anti-tissue factor (TF) (left panel) and anti-factor VII(fVII) (right panel) from fractionated samples of plasma tissue factor (pTF) taken from three locations in a representative patient (one of three). Molecular standards appear at the left in each panel. Plasma TF from each location was separated as described (16) into microparticle (mpTF) and supernatant (spTF) fractions. Note that all TF proteins migrate at 47.5 kDa except for recombinant soluble TF (rTF). The highest amount of TF is in the supernatant fraction of wound pTF. In contrast, the microparticle fraction of wound pTF contains the largest amount of fVII/fVIIa complex (right panel).