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Figure 1 Specificity of cleavage reaction for rat plasminogen activator inhibitor type 1 (PAI-1) messenger ribonucleic acid (mRNA) by E2 deoxyribonucleic acid (DNA) enzyme. (a) E2 cleaved the 24-base oligonucleotide S2, derived from the sequence of rat PAI-1 mRNA, in a dose- and time-dependent manner. (b) E2 also cleaved a rat PAI-1 mRNA transcript in a dose-dependent manner to give the 156 nucleotide cleavage product. Neither the scrambled control E0 nor E3 cleaved the rat PAI-1 mRNA transcript. (c) Single nucleotide substitution between rat and human PAI-1 mRNA target sequence prevents cleavage of rat PAI-1 mRNA transcript by E3.