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Figure 1 Detection by reverse transcription (RT) polymerase chain reaction of human _1A-adrenoceptors (_1A-ARs), ß₁-adrenoceptors (ß₁-AR), ß₂-ARs, and ß₃-ARs, and hormone-sensitive lipase (LIPE) gene transcripts in human internal mammary artery (IMA) and white adipose tissue. Amplified cDNA fragments were separated on a 2% agarose gel and visualized by staining with ethidium bromide. Sizes of the polymerase chain reaction products are indicated on the middle of the panels. This experiment is representative of three investigations performed on three vessels obtained from different patients. ADNg = genomic DNA; AR-AS = reverse strand; AR-S = forward strand; hfat = human fat; M = 100 pb ladder (Biolabs, New England); NRT = none RT.