Click on image to view larger version.

Figure 3 HIF-1(+) transgenic hearts show increased expression of HIF-1, VEGF, and iNOS in the peri-infarct region compared to control HIF-1(–) hearts. (A) Western blot analysis of HIF-1, VEGF, and iNOS in infarct, peri-infarct, and remote left ventricular regions at 24 h and 4 weeks after LAD ligation and in sham-operated hearts. + = HIF-1(+) hearts; –= HIF-1(–) hearts. (B) Immunohistochemical localization of HIF-1, VEGF, and iNOS protein in peri-infarct areas at 4 weeks in HIF-1(+) and HIF-1 (–) hearts. Sections were counterstained with hematoxylin. Scale bar = 80 µm.