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Figure 3 Immunohistochemistry for tissue factor (TF), alternatively spliced human tissue factor (asHTF), and desmin. Polyclonal rabbit antibodies directed against the extracellular domain of TF and the unique C-terminal end of asHTF were used for staining of the myocardial biopsies obtained from a patient with normal ejection fraction (EF) (64%) and a patient with dilated cardiomyopathy (DCM) (21%). (A and B) Cardiomyocytes and endothelial cells within the normal myocardium stained positive for asHTF. Original magnification 400x. (C) Immunohistochemistry for desmin showed positive staining of Z-bands of the cardiomyocytes in a patient with normal EF. The sarcolemma and Z-bands of the same patient were also positive for TF (arrows, D). Original magnification 630x. (E) The Z-bands stained also positive for desmin in the myocardium of a patient with DCM. In contrary to desmin, TF staining of the Z-bands was faint or completely absent in the myocardial biopsy of the same DCM patient (arrows, F and G). Myocardial TF was located within the cytosol around the nucleus in DCM (arrow heads, F and G). Original magnification 630x. (H) Control for TF staining. Original magnification 630x. (I) TF staining of the Z-bands of a patient with normal EF without hematoxylin. Original magnification 630x. (J) Immunohistochemistry for TF showed a diffuse staining with absence of TF-positive Z-bands. TF was located around vacuoles (arrowheads). Original magnification 630x.