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Figure 2 Speckle tracking of successive two-dimensional images obtained from a speckle tracking imaging movie at the apical level during systole in a representative patient. Shown is every sixth frame from successive apical short-axis images, from end-diastole (ED) (onset of QRS of the electrocardiogram) to end-systole (ES), as displayed on the workstation. The small red dots depict the endocardial (Endo) and epicardial (Epi) borders, with the midmyocardium shown more boldly, defining the region of interest. The colored points track individual locations throughout the cardiac cycle. The left ventricular (LV) centroid is shown as a small red cross in the LV cavity and is determined from the midmyocardial line in each frame. The blue crossed lines indicate the averaged LV rotation calculated from the angle displacement of all of mid-myocardial dots.