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Figure 6 Phosphatidyl-inositol-3-kinase (PI3K) is a downstream target of endothelial SH2-domain containing tyrosine phosphatase-1 (SHP-1). (A) Inhibition of PI3K activity by wortmannin (10 nmol/l) abolished the increased O2·– production after treatment with SHP-1 antisense oligodesoxynucleotide (AS-ODN) or sodium stibogluconate (SS). (B) Co-staining of immunoprecipitates of the p85 regulatory subunit of PI3K showed increased tyrosine phosphorylation of p85 after knock out of SHP-1. Means of three independent experiments are shown. *, **Significantly different vs. random ODN at p < 0.05 or p < 0.01, respectively; #, ##significantly different vs. SHP-1 AS/SS at p < 0.05 or p < 0.01, respectively.