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Figure 3 Degradation of SH2-domain containing tyrosine phosphatase-1 (SHP-1) protein increases endothelial O2·– production. Human umbilical vein endothelial cells O2·– release was enhanced, when SHP-1 was inhibited by a pharmacological agent (sodium stibogluconate [SS] 10 µg/ml) and when its protein was degraded by antisense oligodesoxynucleotide (AS-ODN) or short interfering ribonucleic acid (siRNA). N-Acetylcysteine (NAC) reduced O2·– production. *, **Significantly different vs. control at p < 0.05 and p < 0.01, respectively.