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Figure 3 (A) Forward scatter (FSC) and side scatter (SSC) analysis of microparticles in human plasma. The section containing microparticles was gated using a size <1.5 µm in FSC-SSC scatter using standard beads (bead size = 4.2µm). (B) A histogram analysis of CD144-expression in the gated microparticles in human plasma. The thin line represents control-IgG and the thick line represents CD144. (n = 5) (C to D) Representative dot-plot graphs of microparticles expressing CD144-CD42b in human plasma in patients with or without DM. The lower right section of each graph contains EMP as CD144-positive and CD42b-negative microparticles. DM = diabetes mellitus; other abbreviations as in Figures 1 and 2.