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Figure 4 Immunostaining (serial sections) for collagen I (A), MMP-1 (B), and TIMP-1 (C) in myocardium of patients with the lowest ejection fraction demonstrating that the amount of TIMP-1 is obviously less in comparison with collagen I and MMP-1. Collagen I is red, MMP-1 and TIMP-1 are green, nuclei are blue. (D) Quantitative analysis of collagen I per tissue area demonstrates significantly increased levels of fibrosis in patients with decompensated hypertrophy. Abbreviations as in Figure 1.