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Figure 1 (A) The experimental protocol. The tissue perfusion unit (TPU), pH, and temperature were serially measured at baseline, 30 and 60 min after coronary occlusion, after 30 and 60 min of exposure, 30 min after ultrasound (US) was stopped, and 15 min after opening the coronary artery by release of the vascular loop. (B) Schematic shows the method of coronary occlusion of the left anterior descending coronary artery (LAD) diagonal (D) branch and site of ultrasound application, as well as TPU, pH, and temperature measurement. USD = ultrasound.