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Figure 6 (A) Protein expression of eNOS in aortic rings of singularized sedentary mice (n = 5) compared with moving mice (n = 5) and (B) of moving mice (n = 4) compared with moving mice that underwent an exercise program (n = 4). Singularization resulted in a significant reduction of eNOS protein expression (A, p < 0.01), whereas exercise had no effect on eNOS protein expression in moving mice (B, p > 0.05, unpaired two-tailed Student t test).