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Figure 6 Representative tight-seal whole-cell voltage clamp recordings from HEK-293 cells transfected with either KCNH2 or KCNH2 T>C cDNA. The upper trace illustrates the voltage clamp protocol while the middle and lower tracings show WT and T>C currents, respectively. Tail currents are denoted by the arrows. Note the complete absence of KCNH2 tail current in the KCNH2-T>C tracing. The inset shows a series of depolarizations from –70 to 40 mV for a cell expressing WT channels. The scale bar applies to the single traces and is 200 pA by 500 ms.