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Figure 2 Example of flow cytometry showing histograms of red fluorescent intensity for perfluorocarbon-exposed sonicated dextrose albumin microbubbles incubated with R-phycoerythrin–labeled control monoclonal antibody (A), with R-phycoerythrin anti-mouse alone (B), and with serum and R-phycoerythrin–labeled monoclonal antibody to complement C3b, both at baseline (C) and after intralipid infusion (D). Note that incubation of microbubbles and anti-human C3b in the presence of serum drawn in the setting of hypertriglyceridemia resulted in a shift of the curve to the right, reflecting an increase in the mean fluorescent intensity.