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Figure 6 Effects of 7ND overexpression on ischemia-induced neovascularization. (A) Postmortem angiograms at day 21; 7ND overexpression eliminated the development of angiographically visible collateral vessels (top), and angiographic score was significantly lower in 7ND-treated mice as compared with mock-transfected mice (bottom). Bar = 1 x SD (n = 12). (B) Capillary density of the thigh adductor muscle at day 21. Immunohistostaining for CD31, an endothelial cell marker (stained as brown), was performed to determine the capillaries in the ischemic muscle (top). Capillary density at day 21 was significantly reduced by 7ND gene transfer in the ischemic hindlimb (bottom). Bar = 1 x SD (n = 12).