Click on image to view larger version.

Figure 3 Immunohistology for hepatocyte growth factor (HGF) at x400 magnification. (A) Sections obtained from the anterior region adjacent to the scar (top) and that obtained from the remote control posterior region (bottom) were shown. Naked HGF plasmid infusion with ultrasound-mediated microbubble destruction (US/MB) (HGF-US/MB) exhibited positive staining of HGF in both regions. Hepatocyte growth factor stain was negative in control plasmid infusion with US/MB (pVax1-US/MB) and was limited to perivascular regions in naked plasmid HGF infusion during insonation (HGF-US) and naked HGF plasmid infusion without US/MB (HGF-alone) in both regions. Bar graphs compare the score of the magnitude of HGF expression among the groups for the regions adjacent to the scar (B) and for the remote non-infarcted regions (C). *p < 0.05 versus pVax1-US/MB, HGF-US, and HGF-alone.