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Figure 2 (A) Representative recordings of intermittent short-axis images during myocardial contrast echocardiography after coronary occlusion at pulsing interval (PI) of every cardiac cycle (PI = 1) and of every 4th end systole (PI = 4). Arrows denote the risk area. (B) The first frame of the three-frame burst during hepatocyte growth factor gene transfer with ultrasonic microbubble destruction is shown (top) with an illustration (bottom).