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Figure 4 Effect of anti-glycoprotein (GP) IIb/IIIa agents on the dissolution of platelet thrombi formed on the collagen surface. The experiments were performed as described in the legend for Figure 1B. Blood anticoagulated with Argatroban either containing or not containing anti-GP IIb/IIIa (abciximab: 10 µg/ml, eptifibatide: 0.5 µM, tirofiban 0.5 µM) was perfused on the collagen surface on which platelet thrombi had already formed as a result of previous perfusion of control blood. The three-dimensional projection images of the platelet thrombi (the projection images from the top [A], 45° position from the x axis [B], and the side [C]) before and after the second perfusion of blood containing (II) or not containing one of the anti-GP IIb/IIIa agents of abciximab (I) are shown in the left and right panel of I and II. The results are representative of those of eight replicated experiments. No differences in effects were observed among the three anti-GP IIb/IIIa agents tested. No differences in the effects of anti-GP IIb/IIIa agents were observed when blood anticoagulated with heparin was used. For the accompanying videos corresponding to Figure 4 (Videos 4, 5, 6, and 7), please see the July 21 issue of JACC at www.cardiosource.com/jacc.html.