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Figure 3 Effects of anti-glycoprotein (GP) IIb/IIIa agents on three-dimensional platelet thrombus growth on the collagen surface. Experiments were performed as described in the legend for Figure 2, but the platelet thrombi were visualized by laser confocal microscopy. The projection images from the top (A), 45° position from the horizontal axis (B), and the side (C) are shown. Three-dimensional platelet thrombus growth occurred in the control group (left panel), whereas only a single layer of attached platelets was seen in the presence of any of the anti-GP IIb/IIIa agents tested (10 µg/ml abciximab, 0.5 µM eptifibatide, and tirofiban). The results shown are representative of the results of eight replicated experiments. The results with eptifibatide were similar to those obtained with abciximab and tirofiban, but are not shown because of space limitation. There were no differences in the results when the blood was anticoagulated by heparin. For the accompanying videos corresponding to Figure 3 (Videos 1, 2, and 3), please see the July 21 issue of JACC at www.cardiosource.com/jacc.html.