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Figure 2 Patch-clamp and Western blot analyses of the KCNH2 mutant A561T. (A) Representative currents recorded from HEK 293 cells transfected with wild-type (WT) KCNH2 and A561T mutant, as indicated. The KCNH2 currents were activated by depolarizing steps between –70 and 60 mV from a holding potential of –80 mV, and tail currents were recorded on repolarizations to –50 mV. (B) Western blot analysis of KCNH2 channel proteins of wild-type KCNH2 and A561T mutant. The solid and dashed arrows indicate immature and mature forms of KCNH2 protein, respectively.