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Figure 4 Myocardial sections from control and treated hearts stained by TUNEL and propidium iodide. Myocytes and endothelial cells were selectively identified by specific anti-desmin (C and F) and von Willebrand (D and G) labeling, respectively. Non-ischemic control hearts exhibit no yellow TUNEL-positive cells (A and B). In ischemic/reperfused (I/R) control hearts, TUNEL-positivity was consistently observed both in cardiomyocytes (C) and endothelial cells (E). In vivo treatment with minocycline (Mnc) significantly reduced the magnitude of apoptosis in both cell types (F and H). Original magnification: x400.