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Figure 6 Proliferation and survival of parental and cardiotrophin-1 (CT-1)–transferred myoblasts in media containing 10% (A) or 0% (B) serum. Cell number was determined by the absorbance of the WST-8 reagent. Optical density values were normalized to the mean value of each group at 0 h. Data are presented as mean ± SEM of four measurements. *p < 0.05 and **p < 0.01 vs. parental C2C12 cells at same time point by analysis of variance and Bonferroni's multiple-comparison t test. C2C12_CT-1 = CT-1–transferred C2C12 cells.