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Figure 4 Proteasome subunit 20S 5 (green). Counterstain for actin is red; nuclei are blue (TOTO). (A, B) Normal myocardium. (A) Numerous nuclei are labeled for 20S 5. (B) Comparison with the nuclear stain TOTO confirms that the labeled structures are nuclei. (C, D) Human hibernating myocardium. (C) Only few structures are labeled for 20S 5. (D) TOTO reveals that a great number of nuclei are not stained for 20S 5. Bar = 20 µm.