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Figure 3 Massive TUNEL staining of ischemic and reperfused cardiomyocytes. Representative TUNEL and Haematoxylin Eosin (HE) staining of sections derived from control (CON), 20', 58', and reperfusion (REP) left ventricular samples (40 x magnification). The insets show a higher magnification of the same samples (80 x). TUNEL-positive cells were counted in at least 10 (20 x) fields/sample (CON, <1 ± 2%; ischemia 20', 30 ± 5%; ischemia 58', 50 ± 10%; reperfusion 20 ± 5%). Abbreviations as defined in Figure 1.