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Figure 5 (A, panels a to c) Three consecutive frozen sections of NIH3T3 + hHGF + TK transplanted muscle. (panel a) Immunohistochemical staining for vWF and (panel b) -smooth muscle actin (SMA) and (panel c) elastica van Gieson staining. Scale bars = 100 µm. (B) Maturation of vessels was compared by using three consecutive frozen sections. Most of the von Willebrand factor (vWF)-positive vessels in NIH3T3 + hHGF + TK transplanted mice also stained with -SMA (n = 20; *p < 0.05, p < 0.001, p < 0.01). Abbreviations as in Figure 1.