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Figure 2 (A) Influence of hHGF and/or TK genes on cell growth in vitro. The growth rate of the hHGF-transfected NIH3T3 cells was slightly higher than that of the nontransfected cells, but TK had no effect on cell growth. (circles = NIH3T3; diamonds = NIH3T3 + hHGF; squares = NIH3T3 + hHGF + TK) (n = 3). (B) Growth-inhibitory effect of ganciclovir. The IC₅₀ of ganciclovir for the NIH3T3 + hHGF + TK group (solid bars) was 1,000 times lower than that for the NIH3T3 + hHGF group (open bars) (n = 5). (C) Apoptotic cells stained with annexin V-EGFP at the cell membrane after exposure to ganciclovir. Abbreviations as in Figure 1.