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Figure 5 Adenoviral vascular endothelial growth factor-165 (AdVEGF-165) injection results in increased TUNEL-positive cardiac myocytes in the infarct border zone. Representative TUNEL-stained (red) nuclei in sections from the peri-infarct zone four weeks after injection of (A) 1 million skeletal myoblasts (SKMB), (B) 1 x 107 pfu AdVEGF-165, or (C) 1 million SKMB transfected with 1 x 107 pfu AdVEGF-165 each in five equally divided injections. (D) Number of TUNEL-positive cells (white bars) and TUNEL-positive cardiac myocytes (black bars) per 100 nuclei as quantified in five high power fields (
600 nuclei) from the infarct border zone per animal. Data represent mean ± SD. n = 4 animals per group. p < 0.01 direct viral injection versus transfected SKMB for both Luc and VEGF-165. No significant differences were observed between VEGF-165 and Luc within each delivery strategy.