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Figure 2 (A) Typical autoradiopraph of light-dependent in vitro phosphorylation of rhodopsin (³²P-ROS) by cytosolic (CYT) and membranous (MEM) GRK obtained from the RA from a non-failing (3-year-old male donor; NYHA class 0) and failing (61-year-old male recipient; NYHA class IV) heart. (B) Bar graph showing cytosolic and membranous GRK activity in right atrial appendages obtained from four non-failing (mean age 30 ± 12 years [range 3 to 52]; 3 males and 1 female; NYHA class 0) and four failing (mean age 51 ± 4 years [range 44 to 63]; 4 males; NYHA class IV) hearts. Activities were calculated as ³²P incorporated in rhodopsin in cpm/mg protein. Data are given as the mean value ± SEM. *p = 0.0381 vs. non-failing heart.