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Figure 3 Effect of exogenous tumor necrosis factor- on myocardial endothelial nitric oxide synthase (eNOS) (A) and inducible nitric oxide synthase (iNOS) (B) messenger ribonucleic acid expression: relative optical densities of polymerase chain reaction signals. Data were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) polymerase chain reaction signal. Samples of left ventricular myocardium withdrawn at the end of stabilization [1], after 1-h perfusion with normal Krebs-Henseleit solution [2], and after 1 h of perfusion with tumor necrosis factor- [3].