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Figure 1 Effects of global cardioplegic ischemia on myocardial tumor necrosis factor- (TNF-) (A), endothelial nitric oxide synthase (eNOS) (B), and inducible nitric oxide synthase (iNOS) (C) mRNA expression: relative optical densities of polymerase chain reaction signals. Data were normalized to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) polymerase chain reaction signal. Shown are samples of left ventricular myocardium withdrawn at the end of stabilization [1], after ischemia [2], after ischemia with anti-TNF- antibodies in the cardioplegia [3], and after 90 min of normal nonischemic perfusion [4].