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Figure 4 Photomicrographs of specific B-cell lymphoma 2 protein (Bcl-2) (A to C) and heat shock protein 47 (HSP47) immuno-staining (D to F) in neointima formation from day 4 to day 28 after injury. (A) Strongly cell-bound signaling of Bcl-2 in incipient neointima formation at day 4 (arrow). (B) Luminal prevalence of Bcl-2-positive cells and declining expression in basal regions at day 14. (C) Double staining for OX-62 (dark blue) and Bcl-2 (red) in neointima at day 28 demonstrating persistent luminal prevalence of Bcl-2⁺ dendritic cells (no nuclear counterstaining). (D) Luminal cells adhering to the internal elastic lamina exhibit strong HSP47 labeling at day 4. (E) Heat shock protein 47 signals are maximal at the luminal surface and continuously decrease to basal regions at day 28. (F) Double staining for OX-62 (dark blue) and HSP47 (red) in neointima at day 14. Note dendritic cell-bound neointimal HSP47 expression at the luminal border and sparse HSP47 immunoreactivity without presence of dendritic cells in deep neointimal areas (no nuclear counterstaining). Bar = 30 µm (A to F).