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Figure 3 MMP and TIMP activities in atrial myocardium. (A) Gelatin (MMP-2) and casein (MMP-7) zymogram showing the proteolytic activity in sham-operated (Sh), and infarcted rats in mild (M) or severe (S) heart failure. (B) Western blot of proteins from Sh, M, and S rats probed with anti-MMP-2, anti-MMP-7, and anti-MMP-13 antibodies. Analysis of TIMP-1 and -2 activity by reverse zymography (C) and (D) Western blot performed with anti-TIMP-1, anti-TIMP-2 in Sh, M, and S rats.