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Figure 3 Effect of nebivolol, its 4-keto derivative, and anti-bovine LOX-1 monoclonal antibody (bLOX-1 mAb) on oxidized low-density lipoprotein (ox-LDL)-induced variation of superoxide (O2·–) in bovine aortic endothelial cells (BAECs), in Chinese hamster ovary-K1 (CHO) cells, and CHO-K1 cell line stably expressing bovine LOX-1 (BLOX-1-CHO). Nebivolol (25 µmol/l), its 4-keto derivative (25 µmol/l), and bLOX-1 mAb (30 µg/ml) were preincubated with cells for 30 min at 37°C. The cells were then stimulated with ox-LDL (50 µg protein/ml) for 10 min at 37°C. Results are expressed as mean fluorescence intensity (MFI) and are means ± SD of experiments performed in triplicate in six separate occasions. *p < 0.001 versus ox-LDL.