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Figure 2 (A) effect of increasing amounts of nebivolol, its 4-keto derivative, and atenolol on intracellular concentration of superoxide (O2·–) induced by oxidized low-density lipoprotein (ox-LDL) in bovine aortic endothelial cells. The cells were stimulated with ox-LDL (50 µg protein/ml) for 10 min at 37°C. Results are expressed as mean fluorescence intensity (MFI) and are means ± SD of experiments performed in triplicate in six separate occasions. *p < 0.001 versus control. Open bars = nebivolol; solid bars = 4-keto derivative; striped bars = atenolol. (B) Effect of increasing amounts of nebivolol on the translocation of p47phox and Rac1 to the plasma membrane of human umbilical vein endothelial cells. The cells were preincubated with increasing amounts of nebivolol for 30 min at 37°C and then stimulated with ox-LDL (50 µg protein/ml) for 10 min at 37°C. The figure shows representative blots of six different experiments and the average data obtained by densitometric analysis. Results (means ± SD) are expressed as density in arbitrary units (AU). *p < 0.001 versus control. Solid bars = p47phox; open bars = Rac1.