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Figure 4 This deoxyribonucleic acid ladder indicative of apoptosis was detectable in the genomic deoxyribonucleic acid from the left ventricle. Apoptosis was further increased in the non-infarcted left ventricle of diabetes mellitus (DM)+myocardial infarction (MI) compared with MI mice. Amplification of the engrailed-2 (En-2) gene was performed as an internal control. bp = base pair; M = marker.