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Figure 5 Representative Western blots showing densities of Kv1.4, Kv4.2, and Kv4.3 proteins (A). GAPDH was used as internal control to normalize for differences in loading. Samples were done in duplicates. Histogram depicting levels of Kv1.4 protein levels normalized to GAPDH in 2-, 4-, and 8-month-old transgenic (Tg) and nontransgenic (Ntg) ventricular membrane fractions (B). – = Ntg; + = Tg.