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Figure 4 Effect of lectin-like oxidized low-density lipoprotein receptor (LOX-1) antibody (Ab-LOX-1) on apoptosis and caspase-3 expression in response to ischemia-reperfusion (I-R). The number of apoptotic cells was increased in the I-R group (saline-treated rats) compared with the sham control group. Use of the LOX-1 antibody reduced the number of apoptotic cells. Nonspecific anti-goat immunoglobulin G (IgG) had no effect. Left top panel shows representative examples of apoptotic cells (green color). Left bottom panel is summary of data on the number of apoptotic cells from 10 separate experiments. Caspase-3 activity in the myocardium was also increased in the I-R group (saline-treated rats) compared with the sham control group. Use of the LOX-1 antibody reduced caspase-3 activity. Nonspecific IgG had no effect (right panel).