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Figure 4 Effect of catalase (CAT), glutathione (GSH), NAC, mannitol (MAN), and SOD on myocyte apoptosis. Myocytes were treated with 10^–6 mol/l of doxorubicin in the presence or absence of either catalase (4 x 10⁴ U/l), GSH (15 x 10^–3 mol/l), NAC (10^–4 mol/l), MAN (2 x 10^–2 mol/l), or SOD (1.2 x 10⁵ U/l). Fluorescent-stained nuclei of apoptotic myocytes were analyzed morphologically and expressed as the percentage of total nuclei, as described in the Methods section. Control myocytes were incubated in serum-deprived DMEM without any chemicals (n = 6). *p < 0.0001 vs. control. p < 0.01 vs. doxorubicin. p < 0.0001 vs. doxorubicin.